Fig 1. Fluorescence-based dye-terminator sequencing chemistry. Fluorescent signal in a chromatogram format.

Click on figure to enlarge

Single pass sequencing service
Basic quality service, for quick and economical sequence determination:
 600-800 bases read depending upon sample quality
 Plasmids, PCR products purified or crude PCR mixes
 Universal primers included (M13, T3, T7, SP6, pBluescript, pGEM, etc.)
 Results by E-mail

Additional services:
 Primer synthesis for sequencing your sample
 PCR mix purification

Single-strand sequencing service
Most economical approach to sequencing long fragments (< 7kb) when the data is not intended for publication.
 Sequencing is performed on one strand only
 Plasmids, PCR fragments, Cosmids, BACs
 Primer walking approach
 Contig alignment, edition
 All primers included
 E-mailed results + Complete written report

Sample for DNA sequencing (pdf)

Double-strand sequencing
 Double-strand sequencing
 Plasmids, PCR fragments, Cosmids, BACs
 Walks / shot gun
 All Ambiguities resolved
 Guaranteed results
 Publication quality
 Contig alignment, edition and consensus sequence determination
 All primers included
 E-mailed results + Complete report